Western Blot is a technique used for identifying the abundance of proteins within a sample.
It consists of different phases:
- Sample preparation
- Gel electrophoresis
- Proteins transfer
- Blocking
- Antibody incubation
- Protein detection and visualization
DBA ITALIA supports Western Blot experiments with an extensive product line.
Reagents for sample preparation:
- RIPA Lysis Buffer System
- NP-40
- Alkaline Phosphatase Inhibitors
- 2X Laemmli Sample Buffer
- Laemmli Sample Buffer (odor-free), 2X
- Electrophoresis Sample Buffer, 2X (non-reducing)
- Membrane Protein Extraction Kit
- CNM Compartmental Protein Extraction Kit
- Total Protein Extraction Kit
Protein Quantitation:
Reagents for gel preparation:
- Acrylamide Solution, 40%
- Tris Hydrochloride solution, pH 8.0
- SDS (20% Solution)
- TEMED
- Ammonium persulfate
..or use the ready-to-use Precast Gels!
Electrophoresis Chambers
GenBox Mini Electrophoresis Tank
- Ready-to-use, saves time
- Able to isolate phosphorylated proteins by the level of phosphorylation
- Good separation with sharp bands
Running and Transfer Buffers:
- Running Buffer, 10X
- MES solution
- Towbin, with SDS, 10X
- Towbin, without SDS, 10X
Protein Transfer from Gel to Membrane:
- UltraCruz Nitrocellulose Pure Transfer Membranes, 0.22 μm pore size
- UltraCruz Nitrocellulose Supported Transfer Membranes, 0.45 μm pore size
- UltraCruz PVDF Transfer membrane, 0.45 μm pore size
- UltraCruz Nylon Transfer Membranes, 0.45 μm pore size
Control lysates:
PerfectWestern Container:
Western Blot containers available in 38 different sizes and perfectly fit blots of any size, including whole blots, halp blots, and strips.
Wash buffers and Ponceau solution:
- Ponceaus S solution
- Tris Buffered Saline 10X
- Tween-20
- Phosphate Buffered Saline: 1 L of 1X
- Tris Buffered Saline with Tween 20
- Phosphate Buffered Saline
Blocking reagents for preventing non-specific protein interactions:
- 6*His, His-Tag Monoclonal antibody
- Alpha Tubulin Polyclonal antibody
- Beta Tubulin Polyclonal antibody
- DYKDDDDK tag Polyclonal antibody (Binds to FLAG tag epitope)
- GST Tag Polyclonal antibody
- Beta Actin Monoclonal antibody
- GAPDH Monoclonal antibody, more than 6000 citations!!
- Flexable Antibody Labeling Kits suitable for single or multiplex staining
Chemiluminescent Substrates
- WESTAR ECL Substrates for Western Blotting (two components)
- WESTAR-ONE ECL Substrates for Western Blotting (one-component)
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